acoL [2019-07-25 09:49:11]

acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)

locus: BSU_08090

pI: 5.27

mw: 48.69 kDa

protein length: 458 aa

gene length: 1377 bp

function: acetoin utilization

product: acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)

essential: no

ec: 1.8.1.4

synonyms: yfjH

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.2|Utilization of acetoin]

Gene

Coordinates: 882,266 883,642

The protein

Catalyzed reaction/ biological activity

• Protein N(⁶)-(dihydrolipoyl)lysine + NAD⁺ --> protein N(⁶)-(lipoyl)lysine + NADH (according to UniProt)

Protein family

• class-I pyridine nucleotide-disulfide oxidoreductase family (with [protein|E9BBAE86DF3E536A987179CC394B472F6F710498|PdhD] and [protein|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|LpdV], according to UniProt)

Paralogous protein(s)

• [protein|E9BBAE86DF3E536A987179CC394B472F6F710498|PdhD], [protein|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|LpdV]

[SW|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|2EQ7] (from Thermus thermophilus, 40% identity)

[SW|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|acoA]-[gene|5F953C3C91EB65A031D04A1D91F1CF501F8EFD09|acoB]-[gene|C4B6C3EE560C8BD353FEABB1A607C89C20DC8D34|acoC]-[gene|2ECF05AEE1549861EB3AE62139F0E9DEE0B0F632|acoL]
  description: [pubmed|11274109]

  sigma factors

  • [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL]: sigma factor, [PubMed|11274109], in [regulon|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|706864AAF36684AD5E46F30E9EB76315AB412700|AcoR]: activation, (interaction with [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL]-containing [SW|RNA polymerase]) [ PubMed|11274109], in [regulon|706864AAF36684AD5E46F30E9EB76315AB412700|AcoR regulon]
  • [protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr]: indirect positive regulation, in [regulon|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr regulon]

  regulation

  • induced by acetoin ([protein|706864AAF36684AD5E46F30E9EB76315AB412700|AcoR]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
  • expression of the operon is strongly induced during [SW|biofilm formation] [pubmed|31113899]

  additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|21815947]

additional information

• the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

Mutant

• MGNA-C351 (acoL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2349 NBRP B. subtilis, Japan]
• BKE08090 ([gene|2ECF05AEE1549861EB3AE62139F0E9DEE0B0F632|acoL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGTTTTCACCTGCTT, downstream forward: _UP4_TAATAAAGGAAAAAGCAGGC
• BKK08090 ([gene|2ECF05AEE1549861EB3AE62139F0E9DEE0B0F632|acoL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGTTTTCACCTGCTT, downstream forward: _UP4_TAATAAAGGAAAAAGCAGGC

labs

• [SW|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]

References

• 11274109,10368162,10666464,16428414,12884008,21815947